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Transforming growth factor β-induced epithelial-mesenchymal transition increases cancer stem-like cells in the PANC-1 cell line



Published on:2016-11-28   Views:333

Abstract

The epithelial-mesenchymal transition playsa crucial role in the progression of pancreatic cancer. The aim of this studywas to examine the possible association between the epithelial-mesenchymaltransition and cancer stem-like cells in pancreatic cancer. We usedtransforming growth factor β to induce an epithelial-mesenchymal transition.The proportion of pancreatic cancer stem-like cells was measured and sorted byflow cytometry. The expression of markers was measured by quantitative PCR andWestern blot analysis. Cell cycle distribution was assessed by flow cytometry.We evaluated the migration and invasion activity by Transwell tests. Theproportion of pancreatic cancer stem-like cells was significantly increasedfollowing transforming growth factor β treatment. Cells were sorted in culture,the cancer stem-like cells exhibited a higher degree of epithelial-mesenchymaltransition and demonstrated upregulation of vimentin, a mesenchymal phenotypicmarker, compared to the CD44(-)CD24(-) cells. Pancreatic cancer stem-like cellsexhibited greater invasion and migration activity in vitro compared to theCD44(-)CD24(-) cells. These results suggested a direct link betweenepithelial-mesenchymal transition and cancer stem-like cells in pancreaticcancer.